ELECTRON MICROSCOPY IDENTIFICATION OF MICROSPORIDIA (ENTEROCYTOZOON BIENEUSI) AND CYCLOSPORA CAYETANENSIS FROM STOOL SAMPLES OF HIV INFECTED PATIENTS
نویسندگان
چکیده
منابع مشابه
Genotype identification of Enterocytozoon bieneusi isolates from stool samples of HIV-infected Tunisian patients
The microsporidian species Enterocytozoon bieneusi is a major cause of chronic diarrhea and malabsorption in patients with AIDS. Genotyping was performed on seven E. bieneusi strains for the first time in Tunisia. All the strains were isolated from stool samples of humans with immunodeficiency virus (HIV) infection. Analysis of the ribosomal RNA gene internal transcribed spacer (rDNA ITS) allow...
متن کاملDetection of Enterocytozoon bieneusi (Microsporidia) by polymerase chain reaction (PCR) using species-specific primer in stool samples of HIV patients.
BACKGROUND & OBJECTIVE Microsporidia are obligate intracellular parasites causing infections predominantly in immunocompromised patients. Enterocytozoon bieneusi is the most important microsporidian causing chronic diarrhoea in AIDS patients. The current method used for diagnosing the microsporidia spores is based on light microscopy using stained smears, which do not differentiate spores at sp...
متن کاملIdentification of genotypes of Enterocytozoon bieneusi from stool samples from human immunodeficiency virus-infected patients in Thailand.
We identified genotypes of Enterocytozoon bieneusi from 33 stool samples of Thai human immunodeficiency virus (HIV)-infected adult patients. Genotype D was identified at the highest frequency (36.4%), while genotype E was the second most common (15.1%). Genotypes O and PigEBITS 7, previously found only in pigs, were observed in Thai HIV-infected patients. Phylogenetic analysis supported a zoono...
متن کاملEnterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain.
In this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in Galicia (NW, Spain). The detection of Enterocytozoon bieneusi spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. None of the positive samples for microsporidia in the staining method were amp...
متن کاملDiagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks.
We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sampl...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2004
ISSN: 0255-0857
DOI: 10.1016/s0255-0857(21)02893-0